Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes.
J Clin Microbiol
; 42(12): 5871-4, 2004 Dec.
Article
em En
| MEDLINE
| ID: mdl-15583328
ABSTRACT
Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Reação em Cadeia da Polimerase
/
Técnicas de Tipagem Bacteriana
/
Burkholderia pseudomallei
/
Burkholderia
/
Burkholderia mallei
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article