Emulsan quantitation by Nile red quenching fluorescence assay.
Appl Microbiol Biotechnol
; 67(6): 767-70, 2005 Jun.
Article
em En
| MEDLINE
| ID: mdl-15614556
ABSTRACT
A Nile red fluorescent technique to quantify 20-200 microg ml(-1) of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm(-1) M(-1). Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG-1.
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Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
/
Espectrometria de Fluorescência
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article