Smad3 mediates TGF-beta1 induction of VEGF production in lung fibroblasts.
Biochem Biophys Res Commun
; 327(2): 393-8, 2005 Feb 11.
Article
em En
| MEDLINE
| ID: mdl-15629128
ABSTRACT
Transforming growth factor-beta1 (TGF-beta1) is a key factor in a variety of physiological and pathological processes. Vascular endothelial growth factor (VEGF) is a key angiogenic factor, and vascular change is one of the features of airway remodeling. We examined the effect of TGF-beta1 on VEGF production by fibroblasts from mice lacking expression of Smad2 or Smad3 as well as human lung fibroblasts treated with or without Smad2 or Smad3 siRNA. TGF-beta1 stimulated VEGF production by fibroblasts from Smad2 deficient animals and wildtype animals. In contrast, TGF-beta1 did not affect VEGF production by fibroblasts from Samd3 deficient mice. Similarly, TGF-beta1 failed to stimulate VEGF production by HFL-1 cells treated with Samd3 siRNA but significantly increased VEGF production by the cells treated with Smad2 siRNA. These result suggest that TGF-beta1 stimulation of VEGF production by fibroblasts is regulated by Smad3 but not by Smad2 signaling.
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Base de dados:
MEDLINE
Assunto principal:
Transativadores
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Fator de Crescimento Transformador beta
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Fator A de Crescimento do Endotélio Vascular
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Proteínas de Ligação a DNA
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Fibroblastos
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Pulmão
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article