Neuromedin B is present in lung cancer cell lines.
Cancer Res
; 52(9 Suppl): 2732s-2736s, 1992 May 01.
Article
em En
| MEDLINE
| ID: mdl-1563005
ABSTRACT
Previously, high levels of gastrin-releasing peptide and its mRNA were detected in classic small cell lung cancer cell lines. Here the ability of lung cancer cell lines to synthesize neuromedin B (NMB), a structurally similar mammalian bombesin-like peptide, was investigated. By radioimmunoassay, NMB (0.1-0.7 pmol/mg of protein) was detected in 23 of 33 lung cancer cell lines. In contrast, gastrin-releasing peptide (0.1-12.9 pmol/mg of protein) was detected in 16 of 32 cell lines. Using gel filtration and high pressure liquid chromatography techniques, the main peak of immunoreactive NMB coeluted with synthetic NMB. By Northern analysis, a 0.8-kilobase mRNA species was present, using poly(A) mRNA derived from two of three lung cancer cell lines. Using a more sensitive S1 nuclease protection assay, NMB mRNA was present in most of the 15 lung cancer cell lines examined. These data suggest that NMB may be a regulatory peptide in lung cancer.
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Base de dados:
MEDLINE
Assunto principal:
Neurocinina B
/
Neoplasias Pulmonares
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article