Antigen epitope-expressing cytokines for DNA immunization.
Vaccine
; 23(16): 1966-72, 2005 Mar 14.
Article
em En
| MEDLINE
| ID: mdl-15734069
ABSTRACT
Strategies to enhance the efficacy of DNA vaccination against malignancy remain to be established. In this study, a plasmid expressing a tumor antigen incorporated into the signal peptide of human IL-2 was tested as a DNA vaccine in a murine model system. Results showed that antigen-specific CTL responses were elicited by intramuscular injection of these plasmids. Importantly, compared with a minigene vector expressing the same epitope, the OVA epitope-incorporated, IL-2 expression plasmid vaccination was more effective in protecting mice from OVA-expressing tumor challenge. The improved efficacy appears to result from enhanced antigen presentation as well as the immunostimulatory activity of IL-2. This approach may provide new perspectives in designing cytokine-adjuvant DNA vaccines for clinical applications.
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Base de dados:
MEDLINE
Assunto principal:
Citocinas
/
Vacinas Anticâncer
/
Epitopos
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article