[Comparison between two methods for staining DNA of apoptotic spermatozoa].
Zhonghua Nan Ke Xue
; 11(2): 101-3, 2005 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-15755026
ABSTRACT
OBJECTIVE:
To compare two fluorochrome staining methods for the assessment of sperm quality.METHODS:
Washed sperm cells were incubated in 0, 0.15, or 15 micromol/L camptothecin (CAM), or 0.37 or 3.7 mmol/L genistein (GEN) at 37 degrees C for 4 hours. The sperm cells were analyzed for cycle-independent apoptosis and necrosis by single-stain compared with dual-stain fluorescence microscopy to contrast the relative effectiveness of these two approaches.RESULTS:
The single-stain procedure could not detect the sperm viability differences. In contrast, the dual-stain procedure identified a dosage-dependent decrease in the viability and increased necrozoospermia after topoisomerase inhibitor CAM and GEN treatments. Apoptosis was 2-fold higher with topoisomerase inhibitor treatment.CONCLUSION:
The two topoisomerase inhibitors were associated with increased apoptosis and dosage-dependent necrosis. The data suggested that the dual-stain combination Hoechst 33342/PI was more sensitive than the single Hoechst 33342 stain analysis and permitted quantitative analysis of the apoptosis and necrosis in sperm.
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Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
/
Coloração e Rotulagem
/
Apoptose
Idioma:
Zh
Ano de publicação:
2005
Tipo de documento:
Article