The Rho GTPase activators CNF1 and DNT bacterial toxins have mucosal adjuvant properties.
Vaccine
; 23(20): 2551-6, 2005 Apr 08.
Article
em En
| MEDLINE
| ID: mdl-15780436
ABSTRACT
Cytotoxic necrotizing factor 1 (CNF1) from uropathogenic Escherichia coli belongs to a family of factors activating Rho GTPases. We report the in vivo effects of CNF1 in mice co-fed toxin and the soluble protein antigen ovalbumin (OVA). Similar to cholera toxin, CNF1 elicits adjuvanticity anti-OVA responses, both systemic and mucosal. In contrast, the catalytic inactive mutant CNF1-C866S demonstrated no effects. Using dermonecrotic toxin (DNT), a closely related Rho activating toxin from Bordetella, we discovered that the adjuvant property is within the DNT catalytic domain. Manipulation of Rho proteins thus provides a possible new approach for the development of effective mucosal immunoadjuvants.
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Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
/
Fatores de Virulência de Bordetella
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Transglutaminases
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Adjuvantes Imunológicos
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Imunidade nas Mucosas
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Proteínas rho de Ligação ao GTP
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Proteínas de Escherichia coli
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article