Degranulation of human eosinophils induced by Paragonimus westermani-secreted protease.
Korean J Parasitol
; 43(1): 33-7, 2005 Mar.
Article
em En
| MEDLINE
| ID: mdl-15793357
ABSTRACT
Eosinophil degranulation is considered to be a key effector function for the killing of helminthic worms and tissue inflammation at worm-infected lesion sites. However, relatively little data are available with regard to eosinophil response after stimulation with worm-secreted products which contain a large quantity of cysteine proteases. In this study, we attempted to determine whether the degranulation of human eosinophils could be induced by the direct stimulation of the excretory-secretory products (ESP) of Paragonimus westermani, which causes pulmonary paragonimiasis in human beings. Incubation of eosinophils for 3 hr with Paragonimus-secreted products resulted in marked degranulation, as evidenced by the release of eosinophil-derived neurotoxin (EDN) in the culture supernatants. Moreover, superoxide anion was produced by eosinophils after stimulation of the ESP. The ESP-induced EDN release was found to be significantly inhibited when the ESP was pretreated with protease inhibitor cocktail or the cysteine protease inhibitor, E-64. These findings suggest that human eosinophils become degranulated in response to P. westermani-secreted proteases, which may contribute to in vivo tissue inflammation around the worms.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cisteína Endopeptidases
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Degranulação Celular
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Paragonimus westermani
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Eosinófilos
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article