Rapid quantification and taxonomic classification of environmental DNA from both prokaryotic and eukaryotic origins using a microarray.
FEMS Microbiol Lett
; 245(2): 271-8, 2005 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-15837382
ABSTRACT
A microarray has been designed using 62,358 probes matched to both prokaryotic and eukaryotic small-subunit ribosomal RNA genes. The array categorized environmental DNA to specific phylogenetic clusters in under 9 h. To a background of DNA generated from natural outdoor aerosols, known quantities of rRNA gene copies from distinct organisms were added producing corresponding hybridization intensity scores that correlated well with their concentrations (r=0.917). Reproducible differences in microbial community composition were observed by altering the genomic DNA extraction method. Notably, gentle extractions produced peak intensities for Mycoplasmatales and Burkholderiales, whereas a vigorous disruption produced peak intensities for Vibrionales, Clostridiales, and Bacillales.
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Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
DNA
/
DNA Bacteriano
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Genes de RNAr
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Microbiologia Ambiental
/
Células Eucarióticas
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article