Your browser doesn't support javascript.
loading
Overexpression of anti-apoptotic Mcl-1 in testicular germ cell tumours.
Sano, M; Nakanishi, Y; Yagasaki, H; Honma, T; Oinuma, T; Obana, Y; Suzuki, A; Nemoto, N.
Afiliação
  • Sano M; Department of Pathology, Nihon University School of Medicine, Tokyo, Japan. msano@med.nihon-u.ac.jp
Histopathology ; 46(5): 532-9, 2005 May.
Article em En | MEDLINE | ID: mdl-15842635
ABSTRACT

AIMS:

To determine the expression of Mcl-1 in testicular germ cell tumours in order to clarify the role of this anti-apoptotic factor in these tumours. Various members of the Bcl-2 family have been implicated in the apoptotic mechanisms regulating germ cell apoptosis. Mcl-1 is an anti-apoptotic Bcl-2 family member and has recently been reported to be related to the progression of malignancy; however, the involvement of Mcl-1 in the development of germ cell tumours is still unknown. METHODS AND

RESULTS:

Mcl-1 expression in testicular germ cell tumours was investigated by immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR). By immunohistochemistry, overexpression of Mcl-1 was present in all germ cell tumours that were studied, including embryonal carcinoma and yolk sac tumour, as well as choriocarcinoma and teratoma. In teratomas, Mcl-1 was widely distributed in the epithelial, myogenic, neural and mesenchymal components. RT-PCR analysis after microdissection revealed high levels of Mcl-1 mRNA in all tumour variants compared with non-neoplastic germ cells.

CONCLUSION:

Overexpression of anti-apoptotic Mcl-1 may function to enhance the viability of testicular germ cells, thereby leading to tumorigenesis.
Assuntos
Buscar no Google
Base de dados: MEDLINE Assunto principal: Neoplasias Testiculares / Germinoma / Proteínas Proto-Oncogênicas c-bcl-2 / Proteínas de Neoplasias Idioma: En Ano de publicação: 2005 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Neoplasias Testiculares / Germinoma / Proteínas Proto-Oncogênicas c-bcl-2 / Proteínas de Neoplasias Idioma: En Ano de publicação: 2005 Tipo de documento: Article