Imaging ex vivo healthy and pathological human brain tissue with ultra-high-resolution optical coherence tomography.
J Biomed Opt
; 10(1): 11006, 2005.
Article
em En
| MEDLINE
| ID: mdl-15847572
ABSTRACT
The ability of ultra-high-resolution optical coherence tomography (UHR OCT) to discriminate between healthy and pathological human brain tissue is examined by imaging ex vivo tissue morphology of various brain biopsies. Micrometer-scale OCT resolution (0.9x2 microm, axialxlateral) is achieved in biological tissue by interfacing a state-of-the-art TiAl2O3 laser (lambda(c)=800 nm, delta lambda=260 nm, and P(out)=120 mW exfiber) to a free-space OCT system utilizing dynamic focusing. UHR OCT images are acquired from both healthy brain tissue and various types of brain tumors including fibrous, athypical, and transitional meningioma and ganglioglioma. A comparison of the tomograms with standard hematoxylin and eosin (H&E) stained histological sections of the imaged biopsies demonstrates the ability of UHR OCT to visualize and identify morphological features such as microcalcifications (>20 microm), enlarged nuclei of tumor cells (approximately 8 to 15 microm), small cysts, and blood vessels, which are characteristic of neuropathologies and normally absent in healthy brain tissue.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Encefálicas
/
Tomografia de Coerência Óptica
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article