Cloning and expression in yeast of a plant potassium ion transport system.
Science
; 256(5057): 663-5, 1992 May 01.
Article
em En
| MEDLINE
| ID: mdl-1585180
ABSTRACT
A membrane polypeptide involved in K+ transport in a higher plant was cloned by complementation of a yeast mutant defective in K+ uptake with a complementary DNA library from Arabidopsis thaliana. A 2.65-kilobase complementary DNA conferred ability to grow on media with K+ concentration in the micromolar range and to absorb K+ (or 86Rb+) at rates similar to those in wild-type yeast. The predicted amino acid sequence (838 amino acids) has three domains a channel-forming region homologous to animal K+ channels, a cyclic nucleotide-binding site, and an ankyrin-like region.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Plantas
/
Potássio
/
Saccharomyces cerevisiae
/
Canais de Potássio
/
Clonagem Molecular
/
Proteínas de Arabidopsis
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article