[In vitro refolding and functional study of soluble HLA-G1-peptide complex].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 21(3): 265-8, 2005 May.
Article
em Zh
| MEDLINE
| ID: mdl-15862135
ABSTRACT
AIM:
To form soluble HLA-G1-peptide complex by refolding in vitro, and to study its immune function.METHODS:
The heavy chain and beta(2m) of sHLA-G1 were expressed as insoluble aggregates in E. coli, and then the two subunits were refolded to form HLA-G1-peptide complex by dilution method in the presence of specific peptide. The refolded product was purified through Sephadex G-75 gel filtration. The purified product was identified by Western blot with mAb W6/32. The function of soluble HLA-G1 was explored from following three aspects, namely, the influences on cytotoxicity of NK cells, on proliferation of T cells in mixed lymphocyte culture and apoptosis of activated T cells.RESULTS:
The refolded complex was recognized by mAb W6/32. It effectively inhibited cytotoxicity of NK cells and proliferation of T cells, and induced apoptosis of activated T cells.CONCLUSION:
The refolding of soluble HLA-G1-peptide complex has been successfully realized in vitro. The complex can inhibit the functions of NK cells and T cells.
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Antígenos de Histocompatibilidade Classe I
/
Renaturação Proteica
/
Antígenos HLA
Idioma:
Zh
Ano de publicação:
2005
Tipo de documento:
Article