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The response of Na+/K+ -ATPase of human erythrocytes to green laser light treatment.
Kassák, P; Sikurová, L; Kvasnicka, P; Bryszewska, M.
Afiliação
  • Kassák P; Division of Biomedical Physics, Faculty of Mathematics, Physics, and Computer Science, Comenius University, Mlynská dolina F1, 842 48 Bratislava, Slovak Republic. pkassak@yahoo.com
Physiol Res ; 55(2): 189-194, 2006.
Article em En | MEDLINE | ID: mdl-15910177
The objective of this study was to investigate the response of Na(+)/K(+)-ATPase of human erythrocytes to green laser irradiation. Effects of green laser light of fluences 9.5-63.3 J.cm(-2) and merocyanine 540-mediated laser light treatment were studied. Isolated erythrocyte membranes (protein concentration of 1 mg/ml) were irradiated by Nd:YAG laser (532 nm, 30 mW) and then incubated in a medium with 2 mM ATP for 30 min. Activity of ATPase was determined colorimetrically by measuring the colored reaction product of liberated inorganic phosphate and malachite green at 640 nm. Contribution of Na(+)/K(+)-ATPase to overall phosphate production was determined using ouabain. A positive effect of green laser light on Na(+)/K(+)-ATPase activity was observed. The dependence of enzymatically liberated inorganic phosphate on light fluence showed a linear correlation (R(2)=0.96, P=0.0005) for all fluences applied (9.5-63.3 J.cm(-2)). On the other hand, MC 540-mediated phototreatment caused a suppression of enzyme activity.
Assuntos
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Base de dados: MEDLINE Assunto principal: ATPase Trocadora de Sódio-Potássio / Eritrócitos / Lasers Idioma: En Ano de publicação: 2006 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: ATPase Trocadora de Sódio-Potássio / Eritrócitos / Lasers Idioma: En Ano de publicação: 2006 Tipo de documento: Article