Transcript profiling in plastid arginine tRNA-CCG gene knockout moss: construction of Physcomitrella patens plastid DNA microarray.
Plant Biol (Stuttg)
; 7(3): 258-65, 2005 May.
Article
em En
| MEDLINE
| ID: mdl-15912445
ABSTRACT
The moss Physcomitrella patens is a newly established model plant that is widely used for the characterization of gene function by targeted gene knockout or over-expression. The target gene disruption occurs in both the nuclear and chloroplast genomes. We applied DNA microarray technology to the P. patens plastid genome for large-scale analysis of transcripts. A microarray was constructed containing 108 DNA fragments to detect all annotated plastid genes. We analyzed the transcript profile in a knockout transformant for the arginine tRNA gene, trnR-CCG, and confirmed previous results that rbcL and psaI transcripts accumulate in similar levels to wild-type moss, and accD transcript level is higher than those of wild-type moss. Additionally, the plastid DNA microarray revealed that most plastid genes were expressed at similar levels in wild-type and transformant mosses. This indicates that trnR-CCG is not essential for the expression of plastid genes.
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Base de dados:
MEDLINE
Assunto principal:
RNA de Transferência
/
Deleção de Genes
/
Plastídeos
/
RNA de Plantas
/
Bryopsida
/
Análise de Sequência com Séries de Oligonucleotídeos
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article