A C-type lectin-like protein from Agkistrodon acutus venom binds to both platelet glycoprotein Ib and coagulation factor IX/factor X.
Biochem Biophys Res Commun
; 332(3): 904-12, 2005 Jul 08.
Article
em En
| MEDLINE
| ID: mdl-15925567
ABSTRACT
Agkisacutacin, a C-type lectin-like protein (CLP) isolated from Agkistrodon acutus venom, had been previously identified as an antagonist of platelet aggregation induced by ristocetin, as well as a certain extent fibrinogenlytic activity. In this study, agkisacutacin was further purified by three-step chromatography, and its biological functions were characterized. Agkisacutacin after further purification retained the effect on ristocetin-induced, von Willebrand factor-dependent platelet aggregation, while it lost the fibrinogenlytic activity. FACS and ELISA assays showed that agkisacutacin belongs to membrane glycoprotein Ib-binding protein (GPIb-bp) for it could block and inhibit the binding of anti-GPIb antibody to GPIb. Especially, agkisacutacin exhibits anti-coagulant activity and the function as IX/X-binding protein was confirmed by PAGE and ELISA. So, agkisacutacin is the first reported CLP that binds to both platelet membrane receptors and coagulation factors.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Fator IX
/
Fator X
/
Complexo Glicoproteico GPIb-IX de Plaquetas
/
Venenos de Crotalídeos
/
Lectinas Tipo C
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article