Determination of DNA base composition by small scale acrylamide-CsCl gradient centrifugation.
J Biochem Biophys Methods
; 63(3): 155-60, 2005 Jun 30.
Article
em En
| MEDLINE
| ID: mdl-15936091
ABSTRACT
In this paper we describe a simple and rapid protocol for DNA base composition determination by CsCl gradient in the presence of acrylamide. This method permits the determination of GC content in microgram amounts of DNA, and results are easily documented in photographs or graphs. The protocol was applied to the characterization of nematode DNA, but can be used for other organisms. Analyzing several experiments the mean standard deviation observed in the calculated GC content is near 1.3%.
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Base de dados:
MEDLINE
Assunto principal:
Composição de Bases
/
Biofísica
/
Centrifugação com Gradiente de Concentração
/
Césio
/
Cloretos
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article