Semi-industrial isolation of salicin and amygdalin from plant extracts using slow rotary counter-current chromatography.
J Chromatogr A
; 1074(1-2): 43-6, 2005 May 13.
Article
em En
| MEDLINE
| ID: mdl-15941037
ABSTRACT
Salicin in the bark extract of Salix alba and amygdalin in the fruit extract of Semen armeniacae were each separated by slow rotary counter-current chromatography (SRCCC). The apparatus was equipped with a 40-L column made of 17 mm i.d. convoluted Teflon tubing. A 500g amount of crude extract containing salicin at 13.5% was separated yielding 63.5 g of salicin at 95.3% purity in 20h using methyl tert-butyl ether-l-butanol (13) saturated by methanol-water (15) as a stationary phase and methanol-water (15) saturated by methyl tert-butyl ether-1-butanol (13) as a mobile phase. A 400g amount of crude extract containing amygdalin at 55.3% was isolated to yield 221.2g of amygdalin at 94.1% purity in 19h using ethyl acetate-1-butanol (12) saturated by water as a stationary phase and water saturated by ethyl acetate-1-butanol (12) as a mobile phase. The flow rate of the mobile phase was 50 ml/min. The results show that industrial SRCCC separation of salicin and amygdalin is feasible using a larger column at a higher flow rate of the mobile phase.
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Base de dados:
MEDLINE
Assunto principal:
Álcoois Benzílicos
/
Extratos Vegetais
/
Amigdalina
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article