Helicobacter pylori specific immune response induced by conservative flagellin linear B-cell epitope.
World J Gastroenterol
; 11(23): 3528-32, 2005 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-15962368
ABSTRACT
AIM:
To testify the immunogenicity of a conservative B-cell linear epitope of Helicobacter pylori (H pylori) flagellin A.METHODS:
Different programs were used to analyze the secondary structure, molecular hydropathy, and surface accessibility of H pylori flagellin A. Linear B-cell epitopes were estimated based on the structural and physiochemical information. Analysis of residue divergence was proposed to screen a conservative linear epitope. The 29-peptide (Pep29mer) synthesized by chemical method, including the predicted conservative B-cell epitope and a known K2d compatible T-cell epitope, was used to immunize mice, and then H pylori-specific antibodies were detected by ELISA.RESULTS:
Based on the analyses of divergent amino acid residues, structural and physiochemical characteristics, it was strongly suggested that the short fragment NDSDGR was the core of a conservative linear epitope in flagellin A. Animals immunized by Pep29mer acquired efficient immune response. In detail, serum H pylori-specific IgA and IgG1 increased significantly in immunized group, while IgG2a only had an insignificant change. H pylori-specific IgA in gastrointestinal flushing fluid also increased significantly.CONCLUSION:
The conservative short fragment NDSDGR is the core of a linear B-cell epitope of flagellin A.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Helicobacter pylori
/
Flagelina
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article