Oral immunization with pBsVP6-transgenic alfalfa protects mice against rotavirus infection.
Virology
; 339(2): 153-63, 2005 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-15992851
ABSTRACT
A critical factor in edible plant-derived vaccine development is adequate expression of the exogenous antigens in transgenic plants. We synthesized a codon-optimized gene (sVP6) encoding the VP6 protein of human group A rotavirus and inserted it into the alfalfa genome using agrobacterium-mediated transformation. As much as 0.28% of the total soluble protein of the pBsVP6-transgenic alfalfa was sVP6. Female BALB/c mice were gavaged weekly with 10 mg of transgenic alfalfa extract containing 24 microg of sVP6 protein and 10 microg of CpG-rich oligodeoxynucleotides as mucosal adjuvant. Immunized mice developed high titers of anti-VP6 serum IgG and mucosal IgA. Offspring of immunized dams developed less severe diarrhea after challenge with simian rotavirus SA-11, indicating that antibodies generated in the dams provided passive heterotypic protection to the pups. These results suggest that oral immunization with pBsVP6-transgenic alfalfa provides a potential means of protecting children and young animals from severe acute rotavirus-induced diarrhea.
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Base de dados:
MEDLINE
Assunto principal:
Infecções por Rotavirus
/
Vacinas Virais
/
Plantas Geneticamente Modificadas
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Rotavirus
/
Proteínas do Capsídeo
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Medicago sativa
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Antígenos Virais
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article