Hyaluronic acid production in Bacillus subtilis.
Appl Environ Microbiol
; 71(7): 3747-52, 2005 Jul.
Article
em En
| MEDLINE
| ID: mdl-16000785
ABSTRACT
The hasA gene from Streptococcus equisimilis, which encodes the enzyme hyaluronan synthase, has been expressed in Bacillus subtilis, resulting in the production of hyaluronic acid (HA) in the 1-MDa range. Artificial operons were assembled and tested, all of which contain the hasA gene along with one or more genes encoding enzymes involved in the synthesis of the UDP-precursor sugars that are required for HA synthesis. It was determined that the production of UDP-glucuronic acid is limiting in B. subtilis and that overexpressing the hasA gene along with the endogenous tuaD gene is sufficient for high-level production of HA. In addition, the B. subtilis-derived material was shown to be secreted and of high quality, comparable to commercially available sources of HA.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
/
Glucuronosiltransferase
/
Ácido Hialurônico
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article