Pathways for clearance of surfactant protein A from the lung.
Am J Physiol Lung Cell Mol Physiol
; 289(6): L1011-8, 2005 Dec.
Article
em En
| MEDLINE
| ID: mdl-16006481
ABSTRACT
Uptake and degradation of (125)I-surfactant protein A (SP-A) over a 1-h period was studied in alveolar cells in culture and in isolated perfused lungs to elucidate the mechanism for clearance of the protein from the alveolar space. Specific inhibitors of clathrin- and actin-dependent endocytosis were utilized. In type II cells, uptake of SP-A, compared with controls, was decreased by 60% on incubation with clathrin inhibitors (amantadine and phenylarsine oxide) or with the actin inhibitor cytochalasin D. All agents reduced SP-A metabolism by alveolar macrophages. Untreated rat isolated perfused lungs internalized 36% of instilled SP-A, and 56% of the incorporated SP-A was degraded. Inhibitors of clathrin and actin significantly reduced SP-A uptake by approximately 54%, whereas cytochalasin D inhibited SP-A degradation. Coincubation of agents did not produce an additive effect on uptake of SP-A by cultured pneumocytes or isolated perfused lungs, indicating that all agents affected the same pathway. Thus SP-A clears the lung via a clathrin-mediated pathway that requires the polymerization of actin.
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Base de dados:
MEDLINE
Assunto principal:
Alvéolos Pulmonares
/
Surfactantes Pulmonares
/
Proteína A Associada a Surfactante Pulmonar
/
Endocitose
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article