Fourier transform infrared reflectance microspectroscopy study of Bacillus subtilis engineered without dipicolinic acid: the contribution of calcium dipicolinate to the mid-infrared absorbance of Bacillus subtilis endospores.
Appl Spectrosc
; 59(7): 893-6, 2005 Jul.
Article
em En
| MEDLINE
| ID: mdl-16053560
ABSTRACT
Mid-infrared spectra of spores of two strains of Bacillus subtilis, PS832 (wild-type) and FB122 (sleB spoVF), that are isogenic except for the two mutations in FB122 were obtained by Fourier transform infrared (FT-IR) reflectance microspectroscopy. The mutations in FB122 cause the spores of this strain to be devoid of dipicolinic acid (pyridine-2,6-dicarboxylic acid; DPA), a biomarker characteristic of bacterial spores. Analysis of these two strains by difference spectroscopy revealed a spectrum similar to that of calcium dipicolinate (CaDPA), a chelate salt of DPA. This difference spectrum was compared to mid-infrared spectra of both DPA and CaDPA, and was attributed to CaDPA only. This is the first report known to the authors of a genetically engineered organism being used to identify the spectral contribution of a particular cellular component.
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Base de dados:
MEDLINE
Assunto principal:
Ácidos Picolínicos
/
Esporos Bacterianos
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Bacillus subtilis
/
Engenharia Genética
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Espectroscopia de Infravermelho com Transformada de Fourier
/
Compostos de Cálcio
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article