Product and product-independent induction of butane oxidation in Pseudomonas butanovora.
FEMS Microbiol Lett
; 250(1): 111-6, 2005 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-16055278
ABSTRACT
Pseudomonas butanovora grows on butane by means of an inducible soluble alkane monooxygenase (sBMO). The induction of sBMO was studied using the wild type and a sBMO reporter strain. The reporter strain has the lacZkan cassette inserted into bmoX, the gene that encodes the alpha-subunit of the hydroxylase of sBMO. The beta-galactosidase activity in the reporter strain was not induced by butane, but was induced by 1-butanol and butyraldehyde. P. butanovora expressed sBMO product-independent activity at 3.0+/-1 nmol ethylene oxide min(-1) mg protein(-1) in stationary phase. The sBMO product-independent activity likely primes the expression of sBMO by butane.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Pseudomonas
/
Butanos
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article