Histone variant macroH2A1.2 is mono-ubiquitinated at its histone domain.
Biochem Biophys Res Commun
; 336(1): 204-9, 2005 Oct 14.
Article
em En
| MEDLINE
| ID: mdl-16129414
ABSTRACT
Histone macroH2A1.2 (macroH2A) is an unusual histone H2A variant with a large non-histone macrodomain at its carboxyl terminal. MacroH2A1.2 is enriched in facultative heterochromatin, including inactivated X chromosomes in mammalian females and senescence-associated heterochromatin foci. We show here that a small population of macroH2A1.2 is mono-ubiquitinated in human HeLa cells. Mass spectrometry analysis revealed that the specific targeting sites for the mono-ubiquitination are Lys115 and Lys116 of the histone domain. A corresponding Lys119 conserved in histone H2A is also mono-ubiquitinated by Ring protein in the polycomb group complex. We suggest that the mono-ubiquitination of macroH2A1.2 and histone H2A has similar or synergistic implications, but that the multiple ubiquitination sites in macroH2A1.2 might confer a variety of functions upon macroH2A1.2 to modulate chromatin states.
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Base de dados:
MEDLINE
Assunto principal:
Histonas
/
Ubiquitina
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article