Simple and effective technical procedures for the packing and application of nanospray PicoFrit HPLC columns for proteomic analyses.
Proteomics
; 5(15): 3864-7, 2005 Oct.
Article
em En
| MEDLINE
| ID: mdl-16130173
ABSTRACT
A simple procedure was developed for packing PicoFrit HPLC columns with chromatographic stationary phase using a reservoir fabricated from standard laboratory HPLC fittings. Packed columns were mounted onto a stainless steel ultra-low volume precolumn filter assembly containing a 0.5-microm pore size steel frit. This format provided a conduit for the application of the nanospray voltage and protected the column from obstruction by sample material. The system was characterised and operational performance assessed by analysis of a range of peptide standards (n = 9).
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article