A novel Arabidopsis gene causes Bax-like lethality in Saccharomyces cerevisiae.
J Biol Chem
; 280(47): 39468-73, 2005 Nov 25.
Article
em En
| MEDLINE
| ID: mdl-16192270
ABSTRACT
Overexpression of the mammalian proapoptotic protein Bax induces cell death in plant and yeast cells. The Bax inihibitor-1 (BI-1) gene rescues yeast and plant from Bax-mediated lethality. Using the Arabidopsis BI-1 (AtBI-1) gene controlled by the GAL1 promoter as a cell death suppressor in yeast, Cdf1 (cell growth defect factor-1) was isolated from Arabidopsis cDNA library. Overexpression of Cdf1 caused cell death in yeast, whereas such an effect was suppressed by co-expression of AtBI-1. The Cdf1 protein fused with a green fluorescent protein was localized in the mitochondria and resulted in the loss of mitochondrial membrane potential in yeast. The Bax-resistant mutant BRM1 demonstrated tolerance against Cdf1-mediated lethality, whereas the Deltaatp4 strain was sensitive to Cdf1. Our results suggest that Cdf1 and Bax cause mitochondria-mediated yeast lethality through partially overlapped pathways.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
Saccharomyces cerevisiae
/
Genes de Plantas
/
Arabidopsis
/
Proteínas de Arabidopsis
/
Proteínas de Membrana
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article