Intracellular FITC-derivatization with PEG.
Electrophoresis
; 26(21): 4204-5, 2005 Nov.
Article
em En
| MEDLINE
| ID: mdl-16217827
ABSTRACT
In order to investigate the amino acids (AAs) in plant cells, we explore an avenue for intracellular derivatization with FITC. In this method, FITC was used to mark AAs in living protoplasts derived from embryogenic calli of common wheat (Triticum aestivum L. c.v. Jinan 177) mediated by PEG. After FITC-derivatization, the AAs in the lysate were determined by CE. The result reveals that this PEG method can be used to transfer FITC into plant cells efficiently, which provides a good method for AA analysis in plant cells.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Protoplastos
/
Fluoresceína-5-Isotiocianato
/
Células Vegetais
/
Aminoácidos
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article