[Molecular identification of medicinal plants: Dendrobium chrysanthum, Dendrobium fimbriatum and their morphologically allied species by PCR-RFLP analyses].
Yao Xue Xue Bao
; 40(8): 728-33, 2005 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-16268508
ABSTRACT
AIM:
To establish a simple method for molecular identification of original plants of D. chrysanthum and D. fimbriatum using molecular marker rDNA ITS region.METHODS:
Restriction patterns of ITS fragments were obtained using PCR-RFLP method. The PCR products of D. chrysanthum and its morphologically allied species were digested at 37 degrees C by Cla I and Apa LI, those of D. fimbriatum and its morphologically allied species were digested by Sph I.RESULTS:
D. chrysanthum, D. fimbriatum and their morphologically allied species could be identified by predicted restriction profiles of PCR-RFLP. The botanical origin of twenty-five fresh samples of "Shihu" collected in markets was identified by this method.CONCLUSION:
The results showed that PCR-RFLP analysis of the rDNA ITS region is a feasible, simple and inexpensive method for determining the botanical origin of the traditional Chinese medicine "Shihu".
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Base de dados:
MEDLINE
Assunto principal:
Plantas Medicinais
/
DNA Ribossômico
/
DNA de Plantas
/
Dendrobium
Idioma:
Zh
Ano de publicação:
2005
Tipo de documento:
Article