Determination of 8-methoxypsoralen in plasma by gas chromatography-mass spectrometry using selected-ion monitoring.
J Chromatogr
; 574(1): 156-60, 1992 Feb 07.
Article
em En
| MEDLINE
| ID: mdl-1629281
ABSTRACT
A sensitive and accurate assay was developed for the measurement of 8-methoxypsoralen in plasma using electron-impact positive-ion mass fragmentography. 4,5,8-Trimethylpsoralen was used as an internal standard. Sample preparation consisted of a two-step liquid phase extraction using acetonitrile and methylene chloride. The calibration curve showed a linear relationship between the peak areas of 8-methoxypsoralen and 4,5,8-trimethylpsoralen over a wide range of 8-methoxypsoralen concentrations (1-500 ng/ml). With-in- and between-run precisions, measured at five different drug concentrations, varied from 0.82 to 1.41% and from 0.82 to 1.86%, respectively.
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Base de dados:
MEDLINE
Assunto principal:
Metoxaleno
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article