Expressed sequence tags from the midgut and an epithelial cell line of Chironomus tentans: annotation, bioinformatic classification of unknown transcripts and analysis of expression levels.
Insect Mol Biol
; 14(6): 689-95, 2005 Dec.
Article
em En
| MEDLINE
| ID: mdl-16313569
ABSTRACT
Expressed sequence tags (ESTs) were generated from two Chironomus tentans cDNA libraries, constructed from an embryo epithelial cell line and from larva midgut tissue. 8584 5'-end ESTs were generated and assembled into 3110 tentative unique transcripts, providing the largest contribution of C. tentans sequences to public databases to date. Annotation using Blast gave 1975 (63.5%) transcripts with a significant match in the major gene/protein databases, 1170 with a best match to Anopheles gambiae and 480 to Drosophila melanogaster. 1091 transcripts (35.1%) had no match to any database. Studies of open reading frames suggest that at least 323 of these contain a coding sequence, indicating that a large proportion of the genes in C. tentans belong to previously unknown gene families.
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Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
Chironomidae
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Proteínas de Insetos
/
Biologia Computacional
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Etiquetas de Sequências Expressas
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Perfilação da Expressão Gênica
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Células Epiteliais
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article