Fast high performance liquid chromatography and ultraviolet-visible quantification of principal phenolic antioxidants in fresh rosemary.
J Chromatogr A
; 1100(1): 20-5, 2005 Dec 23.
Article
em En
| MEDLINE
| ID: mdl-16330281
ABSTRACT
An improved HPLC method is reported for the determination of rosemary's principal phenolic antioxidants, rosmarinic and carnosic acids, providing a fast and simultaneous determination for both of them by using a solid phase column. The analysis was performed with fresh methanolic extractions of Rosmarinus officinalis. To quantify the amount of antioxidants in a fast and reproducible way by means of UV-vis absorption measurements, a spectrophotometric multi-wavelength calibration curve was constructed based on the antioxidant contents obtained with the recently developed HPLC method. This UV-vis methodology can be extended to the determination of other compounds and herbs if the restrictions mentioned in the text are respected.
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Base de dados:
MEDLINE
Assunto principal:
Fenóis
/
Plantas Comestíveis
/
Espectrofotometria Ultravioleta
/
Cromatografia Líquida de Alta Pressão
/
Antioxidantes
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article