Relation between pp65 antigenemia, RT-PCR and viruria for cytomegalovirus detection in kidney transplant recipients.
Transplant Proc
; 37(9): 3768-9, 2005 Nov.
Article
em En
| MEDLINE
| ID: mdl-16386533
ABSTRACT
INTRODUCTION:
We compared the efficiency of detection using pp65 antigenemia, reverse transcription-polymerase chain reaction (RT-PCR), and viruria for the diagnosis of cytomegalovirus (CMV) infections after kidney transplantation. PATIENTS ANDMETHODS:
We evaluated 40 renal transplant recipients between weeks 5 and 12 after transplantation, including 179 blood and 181 urine specimens.RESULTS:
All positive samples by antigenemia were also positive by PCR. However, in 52 cases only PCR was positive (kappa = 0.134 [P < .001]). Viruria was positive in 66 cases, but only 26 were CMV PCR positive. In 34 cases, viruria was negative and PCR positive (P = .192).CONCLUSION:
Detection of DNA in serum is a more sensitive method than antigenemia for the diagnosis of CMV infection. Viruria was not related to the presence of CMV in blood.
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Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
/
Complicações Pós-Operatórias
/
Proteínas da Matriz Viral
/
Transplante de Rim
/
Infecções por Citomegalovirus
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Citomegalovirus
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article