High-level bacterial expression and purification of human SirT2 protein for NMR studies.
Protein Expr Purif
; 48(1): 56-60, 2006 Jul.
Article
em En
| MEDLINE
| ID: mdl-16442310
ABSTRACT
Silent information regulator 2 (Sir2) proteins are a class of protein deacetylase enzymes that play key roles in transcriptional gene silencing, DNA repair, and aging. Here, we describe the high-level bacterial expression and purification of a human SirT2 construct that yields high resolution NMR spectra. By removing the N-terminal helix alpha0 and using Thioredoxin as a fusion partner, greater than 10 mg/L of purified protein can be obtained from minimal media. The protein is fully functional and enables NMR-based screening and structural studies of this important protein.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Sirtuínas
/
Escherichia coli
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article