Drosophila UNR is required for translational repression of male-specific lethal 2 mRNA during regulation of X-chromosome dosage compensation.
Genes Dev
; 20(3): 380-9, 2006 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-16452509
ABSTRACT
The inhibition of male-specific lethal 2 (msl-2) mRNA translation by the RNA-binding protein sex-lethal (SXL) is an essential regulatory step for X-chromosome dosage compensation in Drosophila melanogaster. The mammalian upstream of N-ras (UNR) protein has been implicated in the regulation of mRNA stability and internal ribosome entry site (IRES)-dependent mRNA translation. Here we have identified the Drosophila homolog of mammalian UNR as a cofactor required for SXL-mediated repression of msl-2 translation. UNR interacts with SXL, a female-specific protein. Although UNR is present in both male and female flies, binding of SXL to uridine-rich sequences in the 3' untranslated region (UTR) of msl-2 mRNA recruits UNR to adjacent regulatory sequences, thereby conferring a sex-specific function to UNR. These data identify a novel regulator of dosage compensation in Drosophila that acts coordinately with SXL in translational control.
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Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Biossíntese de Proteínas
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Cromossomo X
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Proteínas Nucleares
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Mecanismo Genético de Compensação de Dose
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Proteínas de Drosophila
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Proteínas de Ligação a DNA
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Drosophila
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article