Quantitative gel electrophoresis of DNA: resolution of overlapping bands of restriction endonuclease digests.
Anal Biochem
; 194(1): 174-84, 1991 Apr.
Article
em En
| MEDLINE
| ID: mdl-1651063
ABSTRACT
Accurate quantitation of the distribution of DNA digested to completion by a restriction endonuclease and separated in an electrophoretic gel is greatly facilitated by electronic imaging systems employing charge-coupled device detectors. Such quantitation permits the extraction of more accurate values of the lengths of both the parent molecular species and the fragments produced by a restriction endonuclease. We describe a method that determines the number of components in bands containing two or more daughter fragments that are of similar length and hence not resolved by gel electrophoresis. We also report a constrained deconvolution procedure based on the equal number of daughter molecules in a terminal digestion by a restriction endonuclease that resolves the distribution of each of the overlapping species in a composite band on a gel and hence increases the accuracy of the lengths assigned to each.
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Base de dados:
MEDLINE
Assunto principal:
DNA
/
Enzimas de Restrição do DNA
/
Eletroforese em Gel de Ágar
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article