Purification and characterization of a beta-glucuronidase present during embryogenesis of the mollusk Pomacea sp.
Protein Pept Lett
; 12(7): 695-700, 2005 Oct.
Article
em En
| MEDLINE
| ID: mdl-16522172
ABSTRACT
A beta-glucuronidase was purified from Pomacea sp. eggs by ammonium sulfate fractionation, DEAE-BioGel and Heparin-Sepharose chromatographies. This enzyme showed a Mr 180 kDa, with subunits of 90 kDa. The kinetic parameters were pH 4.0, temperature 60 degrees C, Km 2.7 x 10(-6) and Vmax 15.3 microM/h, activator Mg+2, and inhibitor lactone of D-saccharic acid. beta-glucuronidase is an exoglucuronidase involved in glycosaminoglycans metabolism with kinetics parameters similar to those found in mammals.
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Base de dados:
MEDLINE
Assunto principal:
Glucuronidase
/
Moluscos
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article