Isolation and bioenergetic characterization of mitochondria from Pichia pastoris.
Yeast
; 23(4): 307-13, 2006 Mar.
Article
em En
| MEDLINE
| ID: mdl-16544272
ABSTRACT
Pichia pastoris is a methylotrophic yeast of high biotechnological interest. The bioenergetic properties of mitochondria from Pichia pastoris have not yet been determined. We report on a protocol for the isolation of the mitochondria in a state that shows good energy coupling. Analysis of Pichia pastoris growth and bioenergetic properties of the isolated mitochondria reveals that glycerol is the carbon source that yields the best results. Under our growth conditions, mitochondria oxidize external NADH but do not possess an alternative oxidase. Finally, Pichia pastoris mitochondria also lack the nucleotide-stimulated uncoupling pathway previously identified in Saccharomyces cerevisiae.
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Base de dados:
MEDLINE
Assunto principal:
Pichia
/
Mitocôndrias
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article