Prokaryotic and eukaryotic monothiol glutaredoxins are able to perform the functions of Grx5 in the biogenesis of Fe/S clusters in yeast mitochondria.
FEBS Lett
; 580(9): 2273-80, 2006 Apr 17.
Article
em En
| MEDLINE
| ID: mdl-16566929
ABSTRACT
The Saccharomyces cerevisiae monothiol glutaredoxin Grx5 participates in the mitochondrial biogenesis of iron-sulfur clusters. Grx5 homologues exist in organisms from bacteria to humans. Chicken (cGRX5) and human (hGRX5) homologues contain a mitochondrial targeting sequence, suggesting a mitochondrial localization for these two proteins. We have compartmentalized the Escherichia coli and Synechocystis sp. homologues, and also cGRX5 and hGRX5, in the mitochondrial matrix of a yeast grx5 mutant. All four heterologous proteins rescue the defects of the mutant. The chicken cGRX5 gene was significantly expressed throughout the embryo stages in different tissues. These results underline the functional conservation of Grx5 homologues throughout evolution.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Saccharomyces cerevisiae
/
Proteínas de Bactérias
/
Galinhas
/
Synechocystis
/
Escherichia coli
/
Mitocôndrias
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article