Isolation and identification of natural endophytic rhizobia from rice (Oryza sativa L.) through rDNA PCR-RFLP and sequence analysis.
Curr Microbiol
; 52(5): 345-9, 2006 May.
Article
em En
| MEDLINE
| ID: mdl-16586025
ABSTRACT
Three novel endophytic rhizobial strains (RRE3, RRE5, and RRE6) were isolated from naturally growing surface-sterilized rice roots. These isolates had the ability to nodulate common bean (Phaseolus vulgaris). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing of 16S rDNA of these isolates revealed that RRE3 and RRE5 are phylogenetically very close to Burkholderia cepacia complex, whereas RRE6 has affinity with Rhizobium leguminosarum bv. phaseoli. Plant infection test using gusA reporter gene-tagged construct of these isolates indicated that bacterial cells can go inside and colonize the rice root interiors. A significant increase in biomass and grain yield was also recorded in greenhouse-grown rice plants inoculated with these isolates.
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Base de dados:
MEDLINE
Assunto principal:
Oryza
/
Polimorfismo de Fragmento de Restrição
/
DNA Ribossômico
/
Reação em Cadeia da Polimerase
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Complexo Burkholderia cepacia
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Rhizobium phaseoli
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article