Virtual histology of transgenic mouse embryos for high-throughput phenotyping.
PLoS Genet
; 2(4): e61, 2006 Apr.
Article
em En
| MEDLINE
| ID: mdl-16683035
ABSTRACT
A bold new effort to disrupt every gene in the mouse genome necessitates systematic, interdisciplinary approaches to analyzing patterning defects in the mouse embryo. We present a novel, rapid, and inexpensive method for obtaining high-resolution virtual histology for phenotypic assessment of mouse embryos. Using osmium tetroxide to differentially stain tissues followed by volumetric X-ray computed tomography to image whole embryos, isometric resolutions of 27 mum or 8 mum were achieved with scan times of 2 h or 12 h, respectively, using mid-gestation E9.5-E12.5 embryos. The datasets generated by this method are immediately amenable to state-of-the-art computational methods of organ patterning analysis. This technique to assess embryo anatomy represents a significant improvement in resolution, time, and expense for the quantitative, three-dimensional analysis of developmental patterning defects attributed to genetically engineered mutations and chemically induced embryotoxicity.
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Base de dados:
MEDLINE
Assunto principal:
Camundongos Transgênicos
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article