Synthetic peptides with selective affinity for apoptotic cells.
Org Biomol Chem
; 4(10): 1966-76, 2006 May 21.
Article
em En
| MEDLINE
| ID: mdl-16688342
ABSTRACT
The appearance of anionic phosphatidylserine (PS) in the outer monolayer of the plasma membrane is a universal indicator of the early/intermediate stages of cell apoptosis. The most common method of detecting PS on a cell surface is to use the protein annexin V; however, in certain applications there is a need for alternative reagents. Recent research indicates that rationally designed zinc 2,2'-dipicolylamine (Zn2+-DPA) coordination complexes can mimic the apoptosis sensing function of annexin V. Here, a series of fluorescently-labelled, tri- and pentapeptides with side chains containing Zn2+-DPA are prepared and shown to selectively bind to anionic vesicle membranes. Fluorescein-labelled versions of the peptides are used to detect apoptotic cells by fluorescence microscopy and flow cytometry.
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Base de dados:
MEDLINE
Assunto principal:
Oligopeptídeos
/
Sondas Moleculares
/
Apoptose
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article