HPLC determination of a novel aroylhydrazone iron chelator (o-108) in rabbit plasma and its application to a pilot pharmacokinetic study.
J Chromatogr B Analyt Technol Biomed Life Sci
; 838(2): 107-12, 2006 Jul 11.
Article
em En
| MEDLINE
| ID: mdl-16725389
ABSTRACT
A high performance liquid chromatographic method for the determination of a biocompatible iron chelator, pyridoxal 2-chlorobenzoyl hydrazone (o-108), in rabbit plasma was developed and validated. The separation was achieved on a C18 column with the mobile phase composed of a mixture of 0.01 M phosphate buffer (pH 6) with the addition of EDTA (2 mM), methanol and acetonitrile (422414; v/v/v). The method was validated with respect to selectivity, linearity (0.8-150 microg/mL), intra- and inter-day variability and stability. This method was successfully applied to the analysis of the samples obtained from a pilot pharmacokinetic experiment, in which the chelator was administered intravenously to rabbits.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Quelantes de Ferro
/
Cromatografia Líquida de Alta Pressão
/
Hidrazonas
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article