[Expression and effects of nuclear distribution C (NUDC) protein in nasopharyngeal carcinoma cell lines].

ABSTRACT

BACKGROUND & OBJECTIVE: Nuclear migration is essential for growth, development, and cellular function of eukaryotes. Nuclear distribution C (NUDC) protein plays an important role in nuclear migration. This study was to detect the expression of NUDC protein in nasopharyngeal carcinoma (NPC) cell lines CNE-2 and HNE-2, and to investigate the effect of anti-NUDC antibody on the growth of both cell lines. METHODS: Soluble fusion protein GST-NUDC was expressed in the cytoplasm of Escherichia coli, and its poly-antibody was prepared. The subcellular localization of NUDC protein in CNE-2 and HNE-2 cells was detected with indirect immunofluorescent staining, the expression of NUDC protein in CNE-2 and HNE-2 cells was detected by Western blot, the effect of anti-NUDC antibody on the growth of CNE-2 and HNE-2 cells was detected by MTT assay. RESULTS: NUDC protein was expressed in both nasopharyngeal non-cancerous tissues and CNE-2 and HNE-2 cells, and its expression was higher in CNE-2 and HNE-2 cells than in nasopharyngeal non-cancerous tissues. NUDC protein in CNE-2 and HNE-2 cells was distributed mainly in the karyolemma of cytoplasm, and in nuclei asymmetrically. Anti-NUDC antibody inhibited the growth of CNE-2 and HNE-2 cells in a dose-dependent manner. CONCLUSIONS: NUDC protein is expressed highly in CNE-2 and HNE-2 cells. Anti-NUDC antibody could inhibit the growth of CNE-2 and HNE-2 cells.