Specific activation of microRNA-127 with downregulation of the proto-oncogene BCL6 by chromatin-modifying drugs in human cancer cells.
Cancer Cell
; 9(6): 435-43, 2006 Jun.
Article
em En
| MEDLINE
| ID: mdl-16766263
ABSTRACT
Expression profiling of T24 cells revealed that 17 out of 313 human miRNAs were upregulated more than 3-fold by simultaneous treatment with the chromatin-modifying drugs 5-aza-2'-deoxycytidine and 4-phenylbutyric acid. One of these, miR-127, is embedded in a CpG island and is highly induced from its own promoter after treatment. miR-127 is usually expressed as part of a miRNA cluster in normal cells but not in cancer cells, suggesting that it is subject to epigenetic silencing. In addition, the proto-oncogene BCL6, a potential target of miR-127, was translationally downregulated after treatment. These results suggest that DNA demethylation and histone deacetylase inhibition can activate expression of miRNAs that may act as tumor suppressors.
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Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
MicroRNAs
/
Proteínas de Ligação a DNA
/
Proteínas Proto-Oncogênicas c-bcl-6
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article