Efficient purification and preconcentration of erythropoietin in human urine by reusable immunoaffinity column.
J Chromatogr B Analyt Technol Biomed Life Sci
; 843(1): 125-30, 2006 Oct 20.
Article
em En
| MEDLINE
| ID: mdl-16790370
ABSTRACT
In this paper, an efficient method is proposed for purification and preconcentration of erythropoietin (EPO) in human urine samples. The EPO-specific immunoaffinity column (IAC) was generated by covalent immobilization of anti-EPO polyclonal antibodies on Sepharose 4B support. The EPO-binding capacity of the IAC was found to be about 2.0 microg (6.6IU) per 1.5 mL of gel and the activity recoveries of EPO at low concentrations of 7.8, 10 and 120 m IU/mL by the IAC were between 78 and 86%. Substantial cleanup effect was observed when the IAC was applied to human urine samples.
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Base de dados:
MEDLINE
Assunto principal:
Cromatografia de Afinidade
/
Eritropoetina
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article