Poly(N,N-dimethylacrylamide)-grafted polyacrylamide: a self-coating copolymer for sieving separation of native proteins by CE.
Electrophoresis
; 27(15): 3086-92, 2006 Aug.
Article
em En
| MEDLINE
| ID: mdl-16838285
ABSTRACT
The potential of a series of newly synthesized poly(N,N-dimethylacrylamide) (PDMA) grafted polyacrylamide (PAM) copolymers (P(AM-PDMA)) as a replaceable separation medium for protein analysis was studied. A comparative study with and without copolymers was performed; the separation efficiency, analysis reproducibility and protein recovery proved that the P(AM-PDMA) copolymers were efficient in suppressing the adsorption of basic proteins onto the silica capillary wall. Furthermore, the size-dependent retardation of native proteins in a representative P(AM-PDMA) copolymer was demonstrated by Ferguson analysis. The results showed that the P(AM-PDMA) copolymers combine the good coating property of PDMA and the sieving property of PAM and could be applied as a sieving matrix for the analysis of native proteins.
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Base de dados:
MEDLINE
Assunto principal:
Polímeros
/
Acrilamidas
/
Proteínas
/
Eletroforese Capilar
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article