A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes.
Proc Natl Acad Sci U S A
; 103(30): 11240-5, 2006 Jul 25.
Article
em En
| MEDLINE
| ID: mdl-16840556
ABSTRACT
Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Biotecnologia
/
Genoma Bacteriano
/
Análise de Sequência de DNA
/
Genes Bacterianos
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article