The influence of medium composition and matrix on long-term cultivation of primary porcine and human hepatocytes.
Biomol Eng
; 24(1): 59-69, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-16870503
The differentiated hepatocyte phenotype remains difficult to maintain in culture. The duration over which phenotypically stable hepatocytes can be cultured ranges from a couple of days to a few weeks. Shortcomings in medium formulation may be a factor in this lack of success. We have investigated effects of medium formulation on primary porcine and human hepatocyte cultures. We tested seven culture medium compositions (DMEM, ExCell 400, HepatoZYME-SFM, L-15 Leibovitz, SF-3, Waymouth, and Williams' E) and the effects of serum, fibronectin and biomatrix in a sandwich culture configuration. Albumin, urea, cholesterol, GOT, GPT, LDH and triglyceride concentrations were measured over 14 days. For both human and porcine cultures, the best results were obtained with SF-3 medium. Cells cultivated with Williams' E medium and FCS had good morphology and synthetic function during the first days of culture. However, continued addition of serum, was associated with a subsequent loss of differentiated phenotype. Addition of fibronectin was associated with improved function in cultures maintained in SF-3 medium whilst biomatrix had no effect. In contrast, addition of fibronectin did not influence cultures maintained in Williams' E medium, but cultures with biomatrix were associated with improved function at longer time points.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura de Células
/
Meios de Cultura
/
Hepatócitos
/
Engenharia Tecidual
/
Matriz Extracelular
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article