Bacteriophage T4 gene 32 protein shares antigen determinants with reconstituted HeLa hnRNP proteins in western blots.
Biochem Biophys Res Commun
; 167(3): 1196-9, 1990 Mar 30.
Article
em En
| MEDLINE
| ID: mdl-1690995
ABSTRACT
A polyclonal antiserum against purified bacteriophage T4 gene 32 protein was raised in rabbits. In Western blots it detected a number of SDS-PAGE separated nuclear and ribosomal proteins of HeLa cells. Using a renaturing blotting system, however, larger hnRNP proteins ranging between 66.000 and 82.000 Da preferentially reacted with this antiserum. In addition hnRNP group A core proteins were detected to a minor extent. Nucleic acid binding proteins like histones or ribosomal proteins were not stained by this antibody after renaturation.
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Base de dados:
MEDLINE
Assunto principal:
Ribonucleoproteínas
/
Fagos T
/
Proteínas Virais
/
Proteínas de Ligação a DNA
/
Epitopos
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article